製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FAM48A
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 98-191 (DSETIRLPYE EGELLEYLDA EELPPILVDL LEKSQVNIFH CGCVIAEIRD YRQSSNMKSP GYQSRHILLR PTMQTLICDV HSITSDNHKW TQED) of Human FAM48A.

  • ポジティブ・コントロール
    • Human pancreas tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by Fgf signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy.
  • 組織特異性
    Highly expressed in testis, moderately in brain and pituitary gland. Expressed in several fetal tissues, including lung, brain, thymus and kidney. Expression is down-regulated in malignant prostate tissues.
  • 配列類似性
    Belongs to the FAM48 family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA667204 antibody
    • AI450544 antibody
    • bA421P11.4 antibody
    • C13 antibody
    • C13orf19 antibody
    • D3Ertd300e antibody
    • FA48A_HUMAN antibody
    • FAM48A antibody
    • FP757 antibody
    • p38-interacting protein antibody
    • p38IP antibody
    • Protein FAM48A antibody
    • RGD1307812 antibody
    • SPT20 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122517 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-FAM48A antibody (ab122517)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing FAM48A with a C-terminal myc-DDK tag (~3.1kDa)
  • ab122517 at 1/400 dilution staining FAM48A in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.

参考文献

ab122517 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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