製品の概要

  • 製品名Anti-FAM3C antibody
    FAM3C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FAM3C
  • アプリケーション適用あり: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Chicken, Cow, Dog, Human, Monkey
    交差が予測される動物種: Mouse, Rat, Chimpanzee, Pufferfish (Fugu), Opossum
  • 免疫原

    Synthetic peptide corresponding to a portion of amino acids 40-80 of Human FAM3C

  • ポジティブ・コントロール
    • Human, mouse and rat brain lysates. Human brain and skin tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-FAM3C antibody 画像

  • All lanes : Anti-FAM3C antibody (ab72182) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate


    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab72182 at 5µg/ml staining FAM3C in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
  • ab72182 at 10µg/ml staining FAM3C in human skin by Immunohistochemistry using paraffin-embedded tissue.
  • ICC/IF image of ab72182 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72182, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FAM3C antibody (ab72182) 使用論文

ab72182 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (prostate)
Specification prostate
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 Dec 08 2011

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount 50 µg
Specification Mouse mammary cell
Treatment 5ng/ml TGF beta for different time periods (hrs)
Gel Running Conditions Reduced Denaturing (10.0% gel)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 31 2011

Application Immunocytochemistry
Sample Human Cell (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate)
Loading amount 25 µg
Specification Prostate
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 Apr 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"