製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 26 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin.
  • 組織特異性Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis.
  • 配列類似性Belongs to the FAM3 family.
  • 翻訳後修飾2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46.
  • 細胞内局在Secreted. Localized in discrete vesicular and perinuclear structure.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-21 antibody
    • C21orf11 antibody
    • C21orf76 antibody
    • Cytokine like protein 2 21 antibody
    • Cytokine-like protein 2-21 antibody
    • Fam3b antibody
    • FAM3B_HUMAN antibody
    • Family with sequence similarity 3 member B antibody
    • ORF9 antibody
    • Pancreatic-derived factor antibody
    • PANDER antibody
    • PRED44 antibody
    • Protein FAM3B antibody
    see all

Anti-FAM3B antibody 画像

  • Anti-FAM3B antibody (ab88142) at 1 µg/ml + immunogen (recombinant full length protein with proprietary tag of 26kDa) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 26 kDa
  • Anti-FAM3B antibody (ab88142) at 5 µg/ml + Human kidney tissue lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 26 kDa
  • All lanes : Anti-FAM3B antibody (ab88142) at 5 µg/ml

    Lane 1 : FAM3B-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat anti-Mouse IgG at 1/2500 dilution
    Lane 2 : Goat anti-Mouse IgG at 1/2500 dilution


    Predicted band size : 26 kDa

Anti-FAM3B antibody (ab88142) 使用論文

ab88142 has not yet been referenced specifically in any publications.

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It is mostly correct, but for 293T cells, the antibody needs to be used at a concentration of at least 5ug/ml (1/200) in order to develop even a weak signal. The datasheet shows an image of a 2...

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