製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FAM38B
  • 特異性
    Specific to isoforms 1, 2, 3 and 4.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide: AGNSTESSKT PVTIEKIYPY YVKAPSDSNS KPIKQLLSEN NFMDITIILS, corresponding to amino acids 2503-2552 of Human FAM38B

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 318 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

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  • Anti-FAM38B antibody (ab81327) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 318 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Isoform 3 has predicted MW 81 kDa.

    Gel concentration: 12%

プロトコール

参考文献

ab81327 has not yet been referenced specifically in any publications.

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