製品の概要

  • 製品名
    Anti-FAM184A antibody
  • 製品の詳細
    Rabbit polyclonal to FAM184A
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 262-365 of Human FAM184A.

  • ポジティブ・コントロール
    • Human heart muscle tissue; HEK293T cell FAM184A over-expression lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

Anti-FAM184A antibody 画像

  • ab122215, at 1/300 dilution, staining FAM184A in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
  • All lanes : Anti-FAM184A antibody (ab122215) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : FAM184A over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • Immunohistochemical analysis of Human umbilical vein endothelial cells, staining FAM184A with ab122215.

    Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 2% BSA for 45 minutes at 25°. Samples were incubated with primary antibody (5 µg/ml in diluent) for 1 hour at 25°C. A Cy3®-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

Anti-FAM184A antibody (ab122215) 使用論文

ab122215 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human umbilical vein endothelial cells)
Specification
human umbilical vein endothelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - triton X
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Nov 01 2012

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount
50 µg
Specification
HeLa and RPE-1
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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