Rabbit polyclonal to FAM178A
antigen sequence: SSDSWEPTSA GSKQNKFPEK RKRNSVDSDL KSTRESMIPK ARESFLEKRP DGPHQKEKFI KHIALKTPGD VLRLEDISKE PSDE, corresponding to internal sequence amino acids 316-399 of Human FAM178A.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Widely expressed. Expressed at higher level in skeletal muscle and at slightly lower level in brain, liver and heart, than in lung, kidney, spleen and thymus.
Belongs to the FAM178 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-FAM178A antibody (ab122480)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122480 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM178A antibody (ab122480)
ab122480 at 1/500 dilution staining FAM178A in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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