製品の概要

  • 製品名Anti-FAM178A antibody
  • 製品の詳細
    Rabbit polyclonal to FAM178A
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: SSDSWEPTSA GSKQNKFPEK RKRNSVDSDL KSTRESMIPK ARESFLEKRP DGPHQKEKFI KHIALKTPGD VLRLEDISKE PSDE, corresponding to internal sequence amino acids 316-399 of Human FAM178A.

  • ポジティブ・コントロール
    • Human cerebellum tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

  • 組織特異性Widely expressed. Expressed at higher level in skeletal muscle and at slightly lower level in brain, liver and heart, than in lung, kidney, spleen and thymus.
  • 配列類似性Belongs to the FAM178 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C10orf6 antibody
    • F178A_HUMAN antibody
    • Fam178a antibody
    • Family with sequence similarity 178, member A antibody
    • Protein FAM178A antibody
    see all

Anti-FAM178A antibody 画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122480 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122480 at 1/500 dilution staining FAM178A in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

Anti-FAM178A antibody (ab122480) 使用論文

ab122480 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions Reduced Denaturing
Loading amount 100000 cells
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jun 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"