Anti-FAM175B 抗体 (ab74333)
Key features and details
- Rabbit polyclonal to FAM175B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FAM175B antibody -
製品の詳細
Rabbit polyclonal to FAM175B -
由来種
Rabbit -
特異性
The antibody recognises a region between residue 350 and 390 of human FAM175B using the numbering given in entry NP_115558.3 (GeneID 23172). -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human FAM175B. Synthetic peptide corresponding to a region between residue 350 and 390 of human FAM175B (NP_115558.3)
Database link: Q15018 -
ポジティブ・コントロール
- WB: Whole HeLa and 293T cell lysate. IP: Whole HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibody was affinity purified using an epitope specific to FAM175B immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74333の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.04 µg/ml. Predicted molecular weight: 47 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
Use a concentration of 0.04 µg/ml. Predicted molecular weight: 47 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1. -
参照データベース
- Entrez Gene: 23172 Human
- Omim: 611144 Human
- SwissProt: Q15018 Human
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別名
- AA589499 antibody
- Abraxas brother 1 antibody
- ABRO 1 antibody
see all
画像
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All lanes : Anti-FAM175B antibody (ab74333) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Predicted band size: 47 kDaDetection: Chemiluminescence with exposure times of 10 seconds
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ab74333 at 1 ug/ml (used for IP at 3 ug/lysate).
Lane 1: Whole HeLa cell lysate at 1 mg for IP (20% of IP loaded).
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74333 は 2 報の論文で使用されています。
- Meyer T et al. Functional characterization of BRCC3 mutations in acute myeloid leukemia with t(8;21)(q22;q22.1). Leukemia 34:404-415 (2020). PubMed: 31576005
- Rabl J et al. Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation. Mol Cell 75:483-497.e9 (2019). PubMed: 31253574