The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa).
FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.