製品の概要

  • 製品名Anti-FAK (phospho Y925) antibody
    FAK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FAK (phospho Y925)
  • 特異性This antibody is specific for FAK only when phosphorylated at tyrosine 925.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human FAK around the phosphorylation site of tyrosine 925 (KVYpEN).

  • ポジティブ・コントロール
    • HepG2 and 293 cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 123 kDa (predicted molecular weight: 119 kDa).
ELISA 1/4000.
ICC/IF Use at an assay dependent concentration. PubMed: 22102809

ターゲット情報

  • 機能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 組織特異性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • ドメインThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻訳後修飾Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 細胞内局在Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Anti-FAK (phospho Y925) antibody 画像

  • All lanes : Anti-FAK (phospho Y925) antibody (ab38512) at 1/500 dilution

    Lane 1 : HepG2 cell lysate, pre-incubated with phosphopeptide.
    Lane 2 : HepG2 cell lysate.
    Lane 3 : 293 cell lysate + EGF + serum.

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L).

    Predicted band size : 119 kDa
    Observed band size : 123 kDa (why is the actual band size different from the predicted?)
  • ab38512 staining FAK (phospho Y925) in murine bone marrow-derived dendritic cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with formaldehyde and blocked with 3% BSA before being incubated with primary antibody. An AlexaFluor®568-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

Anti-FAK (phospho Y925) antibody (ab38512) 使用論文

This product has been referenced in:
  • Gill MB  et al. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J 34:448-65 (2015). Read more (PubMed: 25471072) »
  • Tomioka H  et al. Novel anti-microbial peptide SR-0379 accelerates wound healing via the PI3 kinase/Akt/mTOR pathway. PLoS One 9:e92597 (2014). WB ; Rat . Read more (PubMed: 24675668) »

See all 4 Publications for this product

Product Wall

I am still waiting for the alignment result for ab131349, but our source for ab38512 tells us that it is "designed to react with human FAK phosphorylated at Y925, mouse FAK phosphorylated at Y963, and rat FAK phosphorylated at Y928. It does not react w...

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Thank you for your enquiry. I am sorry to hear you are having a problem with ab38512 (FAK (phospho Y925) antibody). Along with some questions, I would like to suggest the following modifications to your protocol to help increase the detection of FA...

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