FAK overexpression 293T lysate (whole cell) (ab94321)

製品の概要

  • 製品名FAK overexpression 293T lysate (whole cell)
  • 製品の詳細
    FAK overexpression lysate
  • 特記事項ab94321 is a 293T cell transfected lysate in which Human FAK has been transiently over-expressed using a pCMV-FAK plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Domain: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL. The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. Function: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. PTM: Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.

アプリケーション

Our Abpromise guarantee covers the use of ab94321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

FAK overexpression 293T lysate (whole cell) 画像

  • ab94321 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-FAK antibody (ab55632) at 1/500 dilution

    Lane 1 : FAK overexpression 293T lysate (whole cell) (ab94321)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

FAK overexpression 293T lysate (whole cell) (ab94321) 使用論文

ab94321 has not yet been referenced specifically in any publications.

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