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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FAK aa 700-800.
This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab40794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
|WB||1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).Can be blocked with Human FAK peptide (ab211922).|
This image is courtesy of an Abreview submitted by Magali Boissiere (6970246)
Western blot analysis of RAW264.7 cells lysate (40μg/lane) labelling FAK with ab40794 at 1/5000 in 5% Milk PBS Tween for 16 hours at 4ºC. A HRP conjugated goat anti-rabbit poly clonal (1/5000) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"