製品の概要

  • 製品名Anti-FAK antibody [EP695Y]
    FAK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP695Y] to FAK
  • 特異性ab40794 recognises Focal adhesion kinase (FAK).
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FAK aa 700-800.

  • ポジティブ・コントロール
    • WB and ICC: HeLa IHC: human hepatocellular carcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:
    Anti-FAK antibody (HRP) [EP695Y] (ab195196)
    Anti-FAK antibody (Phycoerythrin) [EP695Y] (ab209436)
    Anti-FAK antibody (Alexa Fluor® 405) [EP695Y] (ab210014)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.
IHC-P 1/250.
WB 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).Can be blocked with Human FAK peptide (ab211922).
IP 1/50.

ターゲット情報

  • 機能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 組織特異性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • ドメインThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻訳後修飾Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 細胞内局在Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Anti-FAK antibody [EP695Y] 画像

  • ab40794 staining FAK in PANC-1 cells treated with CCK Octapeptide sulfated (ab120209), by ICC/IF. Increase of FAK expression correlates with increased concentration of CCK Octapeptide sulfated, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120209 (CCK Octapeptide sulfated) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab40794 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution + Hela cell lysate

    Predicted band size : 119 kDa
    Observed band size : 119 kDa
  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.
  • Immunofluorescent staining of HeLa cells using ab40794.
  • The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab40794 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40794, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

Anti-FAK antibody [EP695Y] (ab40794) 使用論文

This product has been referenced in:
  • Dave JM  et al. Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase. J Cell Sci 129:743-56 (2016). IP, IHC-Fr ; Human . Read more (PubMed: 26769900) »
  • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »

See all 23 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH8.6, 100C, 20min
Sample Human Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde
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投稿 Sep 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (MDBK)
Specification MDBK
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton in PBS (20 min)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (MDBK)
Loading amount 5 µg
Specification MDBK
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Nov 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (hippocampus)
Loading amount 25 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil: reactive)
Specification Tonsil: reactive
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
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Mr. Carl Hobbs

Verified customer

投稿 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Cor 1 cells - unstimulated)
Specification Cor 1 cells - unstimulated
Fixative Formaldehyde
Permeabilization Yes - 0.1% TWEEN 20
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 19 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEC)
Specification HUVEC
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton X-100
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jan 20 2010

Thank you for your enquiry. Unfortunately, the exact amino acid sequence information is proprietary and can not be released. However, I can provide you with a 50 amino acid sequence range from where the actual peptide sequence is located within, as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"