製品の概要

  • 製品名Anti-FAK antibody [12G4]
    FAK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [12G4] to FAK
  • 特異性This antibody detects an ~125kDa protein, corresponding to the apparent molecular mass of FAK on SDS-PAGE immunoblots. Since active FAK is highly phosphorylated, its position on WB may vary with its level of phosphorylation.
  • アプリケーション適用あり: Flow Cyt, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    FAK fusion protein (Human, aa 706-1052)

  • ポジティブ・コントロール
    • HeLa Cell Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 125 kDa. This product has also been reported to be suitable for ICC.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • 組織特異性Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • ドメインThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • 翻訳後修飾Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • 細胞内局在Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Anti-FAK antibody [12G4] 画像

  • Overlay histogram showing HeLa cells stained with ab28152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28152, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-FAK antibody [12G4] (ab28152) 使用論文

This product has been referenced in:
  • Yamauchi S  et al. p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion. J Cell Biol 204:1191-207 (2014). Read more (PubMed: 24662565) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (B103)
Loading amount 25 µg
Specification B103
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"