製品の概要

  • 製品名Anti-FADS2 antibody
    FADS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FADS2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Monkey, Baboon, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FADS2.

    (Peptide available as ab90992.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human liver tissue lysate; Human brain tissue lysates; HeLa whole cell lysate; Hep G2 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
  • 組織特異性Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.
  • パスウェイLipid metabolism; polyunsaturated fatty acid biosynthesis.
  • 配列類似性Belongs to the fatty acid desaturase family.
    Contains 1 cytochrome b5 heme-binding domain.
  • 発生段階Found in fetal heart.
  • ドメインThe histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D6D antibody
    • Delta(6) desaturase antibody
    • Delta(6) fatty acid desaturase antibody
    • Delta-6 desaturase antibody
    • DES 6 antibody
    • DES6 antibody
    • FADS 2 antibody
    • FADS2 antibody
    • FADS2_HUMAN antibody
    • FADSD 6 antibody
    • FADSD6 antibody
    • Fatty acid desaturase 2 antibody
    • linoleoyl-CoA desaturase (delta-6-desaturase) like 2 antibody
    • LLCDL 2 antibody
    • LLCDL2 antibody
    • SLL0262 antibody
    • TU 13 antibody
    • TU13 antibody
    see all

Anti-FADS2 antibody 画像

  • All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 62.5 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 45kDa:FADS2.

Anti-FADS2 antibody (ab72189) 使用論文

ab72189 has not yet been referenced specifically in any publications.

Product Wall



We have in our catalog one anti-FADS2, the reference ishttp://www.abcam.com/ab72189.Unfortunately the homology between the immunogen peptide and therainbow trout FADS2 is only 27% (see attachment). It is therefore veryunlikely this antibody i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"