Anti-FADS2 抗体 (ab72189)
Key features and details
- Rabbit polyclonal to FADS2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FADS2 antibody
FADS2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FADS2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey, Baboon, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: Human liver tissue lysate; Human brain tissue lysates; HeLa whole cell lysate; Hep G2 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72189の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa).
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. -
組織特異性
Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index. -
パスウェイ
Lipid metabolism; polyunsaturated fatty acid biosynthesis. -
配列類似性
Belongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain. -
発生段階
Found in fetal heart. -
ドメイン
The histidine box domains may contain the active site and/or be involved in metal ion binding. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9415 Human
- Omim: 606149 Human
- SwissProt: O95864 Human
- SwissProt: Q5REA7 Orangutan
- Unigene: 502745 Human
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別名
- D6D antibody
- Delta(6) desaturase antibody
- Delta(6) fatty acid desaturase antibody
see all
画像
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FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 45kDa:FADS2. -
All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 62.5 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab72189 detects a strong band at 45 kDa in human liver and brain tissue lysates, aswell as hela and hepG2 whole cell lysates. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of Fatty acid desaturase 2 (FADS2). FADS2 has three isoforms, isoform one has a molecular weight of 52 kDa with isoforms two and three a molecular weight of 49 and 45 respectively(SwissProt data). The band we have observed is thought to represent isoform 3 of FADS2 (45 kDa).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab72189 は 4 報の論文で使用されています。
- Cai F et al. Ximenynic Acid Regulation of n-3 PUFA Content in Liver and Brain. Lifestyle Genom 13:64-73 (2020). PubMed: 32036361
- Tsachaki M et al. Impact of 17ß-HSD12, the 3-ketoacyl-CoA reductase of long-chain fatty acid synthesis, on breast cancer cell proliferation and migration. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302749
- Kim W et al. Polyunsaturated Fatty Acid Desaturation Is a Mechanism for Glycolytic NAD+ Recycling. Cell Metab 29:856-870.e7 (2019). PubMed: 30686744
- Naudí A et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . PubMed: 28185952