製品の概要

  • 製品名Anti-FADS1 antibody
    FADS1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to FADS1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
    交差が予測される動物種: Pig
  • 免疫原

    Recombinant fragment: MAPDPVAAET AAQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYNISEFTR RHPGGSRVIS HYAGQDATDP FVAFHINKGL VKKYMNSLLI GELSPEQPS, corresponding to amino acids 1-99 of Human FADS1 (NP_037534) with a 26kDa tag.

  • ポジティブ・コントロール
    • HeLa nuclear extract

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 0.03ng/ml as a capture antibody when used against the immunogen.

ターゲット情報

  • 機能Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively.
  • 組織特異性Expressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina.
  • パスウェイLipid metabolism; polyunsaturated fatty acid biosynthesis.
  • 配列類似性Belongs to the fatty acid desaturase family.
    Contains 1 cytochrome b5 heme-binding domain.
  • 発生段階Highly expressed in fetal liver and fetal brain.
  • ドメインThe histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D5D antibody
    • Delta 5 desaturase antibody
    • Delta 5 fatty acid desaturase antibody
    • Delta(5) desaturase antibody
    • Delta(5) fatty acid desaturase antibody
    • Delta-5 desaturase antibody
    • Fads1 antibody
    • FADS1_HUMAN antibody
    • FADS6 antibody
    • FADSD5 antibody
    • Fatty acid desaturase 1 antibody
    • FLJ38956 antibody
    • Linoleoyl CoA desaturase (delta 6 desaturase) like 1 antibody
    • LLCDL1 antibody
    • TU12 antibody
    see all

Anti-FADS1 antibody 画像

  • Anti-FADS1 antibody (ab77312) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-FADS1 antibody (ab77312) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Detection limit for recombinant tagged FADS1 is approximately 0.03ng/ml as a capture antibody

Anti-FADS1 antibody (ab77312) 使用論文

ab77312 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab77312.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"