Human breast carcinoma tissue; Jurkat cell extracts, treated with PMA (125ng/ml, 30mins).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol Note: without Mg2+ and Ca2+
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Immunogen affinity purified
ab111379 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Contains 1 death domain. Contains 1 DED (death effector) domain.
Contains a death domain involved in the binding of the corresponding domain within Fas receptor.