The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
機能Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
組織特異性Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
配列類似性Contains 1 death domain. Contains 1 DED (death effector) domain.
ドメインContains a death domain involved in the binding of the corresponding domain within Fas receptor.
ab48577 at 4.0ug/ml concentration staining human FADD in epithelial cells of renal tubule by Immunohistochemistry, Paraffin embedded tissue. Arrows indicate positively stained epithelial cells of renal tube.
Anti-FADD antibody (ab48577) 使用論文
has not yet been referenced specifically in any publications.