The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報Peptide ELISA: Antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
関連疾患Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
翻訳後修飾The activation peptide is released by thrombin.
細胞内局在Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.