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Our Abpromise guarantee covers the use of ab4057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 30 minuts at room temperature (ABC method).|
|IHC-Fr||Use at an assay dependent concentration.|
ab4057 staining formalin fixed paraffin-embedded human bowel tumour tissue sections. The sample was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% srum for 1 hour at 24°C, prior to incubation with the primary antibody (diluted 1/100 in milk) for 12 hours at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
ab4057 has not yet been referenced specifically in any publications.