Factor X Human Chromogenic Activity Assay Kit (ab108833)

製品の概要

  • 製品名Factor X Human Chromogenic Activity Assay Kit
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプQuantitative
  • 検出感度
    = 0.125 µg/ml
  • 検出範囲
    0.125 µg/ml - 8 µg/ml
  • 種交差性
    交差種: Human
  • 製品の概要

    Factor X Human Chromogenic Activity Assay Kit (ab108833) is developed to determine human Factor X activity in plasma and cell culture. The assay measures the activation of zymogen Factor X to Factor Xa by RVV. The amidolytic activity of the Factor Xa is quantitated using a highly specific Factor Xa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the Factor X enzymatic activity.

     

    High level of FX activity samples: range of 0.125-8 µg/ml Low level of FX activity samples: range of 0.0156-1 µg/ml

  • 特記事項

    Factor X is a plasma serine protease zymogen involved in the blood coagulation cascade. Factor X is purified from plasma as a two-chain protein consisting of a 45-kDa heavy chain and a 17-kDa light chain. The Factor X heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the Factor X-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue Factor/Factor VIIa) pathway to give an active enzyme Factor Xa. Factor Xa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 機能Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • 組織特異性Plasma; synthesized in the liver.
  • 関連疾患Factor X deficiency
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Factor X Human Chromogenic Activity Assay Kit 画像

  • Factor X Chromogenic Activity measured in cell culture supernatants showing quantity (ug) per mL of tested sample after 40 min of incubation. Values calculated based on Low Level Standard Curve. Samples were diluted 2-10 fold.

  • Factor X Chromogenic Activity measured in biological fluids showing quantity (ug) per mL of tested sample after 10 min of incubation. Values calculated based on High Level Standard Curve. Samples were diluted 2 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

プロトコール

Factor X Human Chromogenic Activity Assay Kit (ab108833) 使用論文

ab108833 has not yet been referenced specifically in any publications.

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I've just received a reply from the lab, and these kits can be analyzed at room temperature. The OD might be slig...

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The Factor VII Human Chromogenic Activity Assay Kit had been developed to measure human FVII activity in plasma and cell cul...

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Thank you for your recent telephone enquiry. I am sorry to confirm that this kit has not been tested in mouse. Due to the nature of the kit, it is difficult to predict whether it would still work on mouse samples. I am sorry we do not have more informa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"