製品の概要

  • 製品名Anti-Factor X antibody
    Factor X 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Factor X
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-P, WB, RIAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human Factor X purified from human plasma

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Flow Cyt
IHC-P
WB
RIA
  • 追加情報 EIA/RIA: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
    • 組織特異性Plasma; synthesized in the liver.
    • 関連疾患Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
    • 配列類似性Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • 翻訳後修飾The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      N- and O-glycosylated.
      The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 製品の状態Cleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
    • 別名
      • Activated factor Xa heavy chain antibody
      • Coagulation factor X antibody
      • F10 antibody
      • FA10_HUMAN antibody
      • factor Xa antibody
      • FX antibody
      • FXA antibody
      • Prothrombinase antibody
      • Stuart factor antibody
      • Stuart Prower factor antibody
      • Stuart-Prower factor antibody
      see all

    Anti-Factor X antibody 画像

    • ICC/IF image of ab79929 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79929 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • IHC image of Factor X staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79929, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Factor X antibody (ab79929) 使用論文

    ab79929 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Fixation Formaldehyde
    Sample Human Cell (hepatocyte)
    Specification hepatocyte
    Permeabilization Yes - 0.1% triton
    Username

    Dr. Xaowen He

    Verified customer

    投稿 Nov 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"