Anti-Factor VIII 抗体 (ab61370)

製品の概要

  • 製品名Anti-Factor VIII antibody
    Factor VIII 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Factor VIII
  • アプリケーション適用あり: Inhibition Assay, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human Factor VIII

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% (v/v)Glycerol/water
  • Concentration information loading...
  • 精製度Ion Exchange Chromatography
  • 特記事項(精製)Salt fractionation followed by ion exchange chromatography.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use at an assay dependent concentration. Binds FVIII/vWF complex in plasma
WB Use at an assay dependent dilution. Predicted molecular weight: 267 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • 関連疾患Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • 配列類似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • ドメインDomain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • 翻訳後修飾Sulfation on Tyr-1699 is essential for binding vWF.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AHF antibody
    • Antihemophilic factor antibody
    • Coagulation factor VIII antibody
    • coagulation factor VIII, procoagulant component antibody
    • coagulation factor VIIIc antibody
    • DXS1253E antibody
    • F8 antibody
    • F8b antibody
    • F8c antibody
    • FA8_HUMAN antibody
    • factor VIII F8B antibody
    • Factor VIIIa light chain antibody
    • FactorVIII antibody
    • FVIII antibody
    • Hema antibody
    • Hemophilia A antibody
    • Hemophilia, classic antibody
    • OTTHUMP00000061446 antibody
    • OTTHUMP00000196174 antibody
    • Procoagulant component antibody
    see all

Anti-Factor VIII antibody (ab61370) 使用論文

ab61370 has not yet been referenced specifically in any publications.

Product Wall

We have not previously used this antibody in rat and so we cannot guarantee that it will work.

I have compared the total homology with rat and there is 46% homology due to some parts of the protein being very well conserved and others which ha...

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Thank you for your inquiry.
This polyclonal must be able to bind FVIII/vWF complex in plasma since it is inhibitory.
However, I do not know if can bind the complex in a western blot application. This hasn't been tested, unfortunately.
I ...

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Thank you for contacting Abcam.

I do not think that you would be able to use the same antibody as both the capture and detection antibody in your sandwich ELISA, as you would want each antibody to detect different portions of the protein.
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"