Anti-Factor V Heavy Chain 抗体 [AHV-5146] (ab61348)

製品の概要

  • 製品名
    Anti-Factor V Heavy Chain antibody [AHV-5146]
    Factor V Heavy Chain 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AHV-5146] to Factor V Heavy Chain
  • 特異性
    This antibody is specific for Human factor V and Va, heavy chain (fragment D, 94,000) of factor Va.
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Factor V Heavy Chain.

  • エピトープ
    Between Arg 306 - Arg 506.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • バッファー
    Preservative: None
    Constituents: 50% (v/v)Glycerol/water
  • Concentration information loading...
  • 精製度
    >95% by SDS-PAGE
  • ポリ/モノ
    モノクローナル
  • クローン名
    AHV-5146
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/2000.
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Factor V is an essential factor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • Activated protein C cofactor antibody
    • Coagulation factor V antibody
    • Coagulation factor V heavy chain antibody

Anti-Factor V Heavy Chain antibody [AHV-5146] 画像

  • Immunofluorescence analysis of pMT2/FV tranfected COS-1 cells, staining Factor V Heavy Chain with ab61348.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/2000 in 0.1% Triton X-100 in PBS) for 1 hour at 37°C. An FITC-conjugated goat anti-mouse polyclonal IgG (1/100) was used as the secondary antibody.

    See Abreview

Anti-Factor V Heavy Chain antibody [AHV-5146] (ab61348) 使用論文

ab61348 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (pMT2/FV tranfected COS-1 cell)
Specification
pMT2/FV tranfected COS-1 cell
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Nov 21 2012

Thank you for contacting us.

The clone number for ab61348 is AHV-5146 and for ab61413 it is AHV-5108.

I will send you the clone nunmber for ab1015 soon.

I hope this information is helpful to you. Please do not hesitate to con...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録