製品の概要

  • 製品名Anti-Factor H antibody
    Factor H 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Factor H
  • 特異性This product gives a single arc when tested by IEP and 2D-IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human Factor H.
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, Double Immunodiffusion, RID, Immunoelectrophoresis, Other, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Horse, Guinea pig, Cat, Dog, Human
    非交差種: Rabbit, Chicken, Pig
  • 免疫原

    Human 155 kDa Factor H purified from plasma.

  • 特記事項


    Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600mg/L for the 155 kDa form and 1-5mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーGlycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Antiserum is prepared by immunisation of sheep with human Factor H and, if necessary, adsorption tomonospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is finally 0.2µm filtered.
  • 一次抗体 備考Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600mg/L for the 155 kDa form and 1-5mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.
  • ポリ/モノポリクローナル
  • ミエローマunknown
  • アイソタイプIgG
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 20813971
Suitability for use in nephelometry and enzyme-linked immunosorbent assays have not been assessed but use in such assays should not necessarily be excluded.
IHC-P Use at an assay dependent concentration. PubMed: 20813971
Flow Cyt Use at an assay dependent concentration. ab37385-Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.
Double Immunodiffusion Use at an assay dependent concentration. 10µL antiserum vs 10µL plasma
RID Use at an assay dependent concentration. (and Rocket IEP) 1µL antiserum/cm² gel vs 5µL neat - 1/10 dilution human plasma.
The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Immunoelectrophoresis Use at an assay dependent concentration. 100µL antiserum vs 5µL plasma
Other Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. PubMed: 22461909

ターゲット情報

  • 機能Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Genetic variations in CFH are associated with basal laminar drusen (BLD) [MIM:126700]; also known as drusen of Bruch membrane or cuticular drusen or grouped early adult-onset drusen. Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch membrane. Basal laminar drusen refers to an early adult-onset drusen phenotype that shows a pattern of uniform small, slightly raised yellow subretinal nodules randomly scattered in the macula. In later stages, these drusen often become more numerous, with clustered groups of drusen scattered throughout the retina. In time these small basal laminar drusen may expand and ultimately lead to a serous pigment epithelial detachment of the macula that may result in vision loss.
    Defects in CFH are the cause of complement factor H deficiency (CFH deficiency) [MIM:609814]. CFH deficiency determines uncontrolled activation of the alternative complement pathway with consumption of C3 and often other terminal complement components. It is associated with a number of renal diseases with variable clinical presentation and progression, including membranoproliferative glomerulonephritis and atypical hemolytic uremic syndrome. CFH deficiency patients may show increased susceptibility to meningococcal infections.
    Defects in CFH are a cause of susceptibility to hemolytic uremic syndrome atypical type 1 (AHUS1) [MIM:235400]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    Genetic variation in CFH is associated with age-related macular degeneration type 4 (ARMD4) [MIM:610698]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性Contains 20 Sushi (CCP/SCR) domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • adrenomedullin binding protein antibody
    • age related maculopathy susceptibility 1 antibody
    • AHUS 1 antibody
    • AHUS1 antibody
    • AMBP 1 antibody
    • AMBP1 antibody
    • ARMD 4 antibody
    • ARMD4 antibody
    • ARMS 1 antibody
    • ARMS1 antibody
    • beta 1 H globulin antibody
    • beta 1H antibody
    • beta1H antibody
    • CFAH_HUMAN antibody
    • CFH antibody
    • CFHL 3 antibody
    • CFHL3 antibody
    • Complement factor H antibody
    • complement factor H, isoform b antibody
    • Factor H antibody
    • factor H like 1 antibody
    • FH antibody
    • FHL 1 antibody
    • FHL1 antibody
    • H factor 1 (complement) antibody
    • H factor 1 antibody
    • H factor 2 (complement) antibody
    • HF 1 antibody
    • HF 2 antibody
    • HF antibody
    • HF1 antibody
    • HF2 antibody
    • HUS antibody
    • MGC88246 antibody
    see all

Anti-Factor H antibody 画像

  • All lanes : Anti-Factor H antibody (ab8842) at 1/500 dilution

    Lane 1 : Purified Human Factor H
    Lane 2 : Human serum sample (albumin depleted)
    Lane 3 : Mouse serum sample (albumin depleted)
    Lane 4 : Rat serum sample (albumin depleted)
    Lane 5 : Guinea pig serum sample (albumin depleted)
    Lane 6 : Cattle serum sample (albumin depleted)
    Lane 7 : Horse serum sample (albumin depleted)
    Lane 8 : Dog serum sample (albumin depleted)
    Lane 9 : Cat serum sample (albumin depleted)

    Developed using the ECL technique

    Performed under non-reducing conditions.

    Image from Bhide MR et al, BMC Res Notes. 2009 Jul 15;2:134, Fig 1.

Anti-Factor H antibody (ab8842) 使用論文

This product has been referenced in:
  • Segers FM  et al. Complement alternative pathway activation in human nonalcoholic steatohepatitis. PLoS One 9:e110053 (2014). Read more (PubMed: 25299043) »
  • Giuntini S  et al. Monoclonal antibodies to meningococcal factor H binding protein with overlapping epitopes and discordant functional activity. PLoS One 7:e34272 (2012). ELISA . Read more (PubMed: 22461909) »

See all 8 Publications for this product

Product Wall

Thanks so much for all your troubleshooting efforts and for including the protocol details and IHC images in the attachement!
I agree this antibody is not working as expected. I would be happy to offer a replacement, credit or refund.
In your...

Read More

DISCOUNT CODE:xx
Expiration date: xx

I am very pleased to hear you would like to accept our offer and test ab8842 in cow. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abre...

Read More

Thank you for submitting your completed questionnaire. I'm sorry to hear this vial of ab8842 is giving you trouble.
To decrease background bands, you might try further dilution of the secondary, to 1:10000, for example. In addition, it might be he...

Read More

Thank you very much for your inquiry and patience while I checked this information.

Indeed, the laboratory has tested this antibody ab8842 via Ouchterlony (double immunodiffusion). In this assay they have not detected any crossreactivity with...

Read More

Thank you for contacting Abcam regarding this. This antibody is produced using purified human H factor (from plasma) as the immunogen. The product is polyclonal as well. Polyclonal antibodies created by introducing the immunogen to the host species and...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (serum)
Specification serum
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Feb 17 2006

Unfortunately this antibody has not been tested with Factor H-like protein-1 (FHL-1) or any of the Factor H related proteins (FHR), we only immunise against total factor H. My feeling is that the product probably will bind FHL-1 and FHR however I cann...

Read More

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abca...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"