製品の概要
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製品名Anti-Factor H antibody
Factor H 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Factor H
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アプリケーション適用あり: WB, ELISA, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide:
HLVPDRKKDQYK
by a Cysteine residue linker, corresponding to internal sequence amino acids 577-587 of Human Factor H -
ポジティブ･コントロール
- Human lung lysate and Human liver tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 98% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab115290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 139 kDa. | |
| ELISA | 1/32000. | |
| IHC-P | Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
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組織特異性Expressed by the liver and secreted in plasma.
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関連疾患Genetic variations in CFH are associated with basal laminar drusen (BLD) [MIM:126700]; also known as drusen of Bruch membrane or cuticular drusen or grouped early adult-onset drusen. Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch membrane. Basal laminar drusen refers to an early adult-onset drusen phenotype that shows a pattern of uniform small, slightly raised yellow subretinal nodules randomly scattered in the macula. In later stages, these drusen often become more numerous, with clustered groups of drusen scattered throughout the retina. In time these small basal laminar drusen may expand and ultimately lead to a serous pigment epithelial detachment of the macula that may result in vision loss.
Defects in CFH are the cause of complement factor H deficiency (CFH deficiency) [MIM:609814]. CFH deficiency determines uncontrolled activation of the alternative complement pathway with consumption of C3 and often other terminal complement components. It is associated with a number of renal diseases with variable clinical presentation and progression, including membranoproliferative glomerulonephritis and atypical hemolytic uremic syndrome. CFH deficiency patients may show increased susceptibility to meningococcal infections.
Defects in CFH are a cause of susceptibility to hemolytic uremic syndrome atypical type 1 (AHUS1) [MIM:235400]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Genetic variation in CFH is associated with age-related macular degeneration type 4 (ARMD4) [MIM:610698]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
配列類似性Contains 20 Sushi (CCP/SCR) domains.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 3075 Human
- Omim: 134370 Human
- SwissProt: P08603 Human
- Unigene: 363396 Human
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別名
- adrenomedullin binding protein antibody
- age related maculopathy susceptibility 1 antibody
- AHUS 1 antibody
see all
Anti-Factor H antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor H antibody (ab115290)Formalin-fixed, paraffin-embedded Human liver tissue labelled with ab115290 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Western blot - Anti-Factor H antibody (ab115290)Anti-Factor H antibody (ab115290) at 0.03 µg/ml + Human lung lysate at 35 µg
Developed using the ECL technique
Predicted band size : 139 kDa
Anti-Factor H antibody (ab115290) 使用論文
ab115290 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"