製品の概要

  • 製品名Anti-Factor B antibody
    Factor B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Factor B
  • 特異性ab72658 detects a fragment from activated Factor B; the Bb fragment, resulting from cleavage adjacent to Lys260.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Sheep, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide:

    KIVLDPSGSMN IYLVLDGSD

    , corresponding to amino acids 260-280 of Human Factor B.

  • ポジティブ・コントロール
    • Extracts from K562 cells, treated with etoposide (25uM, 1hour).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
  • 関連疾患Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 3 Sushi (CCP/SCR) domains.
    Contains 1 VWFA domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AHUS4 antibody
    • B factor properdin antibody
    • BF antibody
    • BFD antibody
    • C3 proaccelerator antibody
    • C3 proactivator antibody
    • C3/C5 convertase antibody
    • CFAB antibody
    • CFAB_HUMAN antibody
    • CFB antibody
    • Complement Factor B antibody
    • Complement factor B Bb fragment antibody
    • FB antibody
    • FBI12 antibody
    • GBG antibody
    • Glycine rich beta glycoprotein antibody
    • Glycine-rich beta glycoprotein antibody
    • H2 Bf antibody
    • H2BF antibody
    • PBF2 antibody
    • Properdin factor B antibody
    see all

Anti-Factor B antibody 画像

  • All lanes : Anti-Factor B antibody (ab72658) at 1/200 dilution

    Lane 1 : Extracts from K562 cells, treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from K562 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 54 and 86 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa (possible non-specific binding),75 kDa. We are unsure as to the identity of these extra bands.

Anti-Factor B antibody (ab72658) 使用論文

ab72658 has not yet been referenced specifically in any publications.

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I am very pleased to hear you would like to accept our offer and test ab72658 in mouse tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this ...

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Thank you for your interest in ab72658. I have confirmed with the laboratory that this antibody is specific for the CFAB Bb which is cleaved at Lys260. It cannot cross react with Factor-Ba. I hope this information is helpful. Please do not hesit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"