製品の概要

  • 製品名Anti-FABP4 (phospho Y20) antibody
    FABP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FABP4 (phospho Y20)
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Rabbit, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FABP4.

    (Peptide available as ab107889.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Adipose (human); Adipose (rat); Mouse Brown adipose tissue. IHC-P: FFPE human lung tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

ターゲット情報

  • 機能Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • 配列類似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメインForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 細胞内局在Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Anti-FABP4 (phospho Y20) antibody 画像

  • IHC image of FABP4 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85875, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-FABP4 (phospho Y20) antibody (ab85875) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : Adult Rat Brown Adipose Tissue Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa


    Exposure time : 16 minutes
  • Anti-FABP4 (phospho Y20) antibody (ab85875) at 1 µg/ml + Adult Mouse Brown Adipose Tissue Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 35 kDa,49 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

Anti-FABP4 (phospho Y20) antibody (ab85875) 使用論文

ab85875 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"