製品の概要

  • 製品名Anti-FABP4 antibody
    FABP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FABP4
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4.

    (Peptide available as ab66681.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Human Adipose Tissue, P7 Mouse Adipose Tissue, Rat Brown Adipose Tissue, Mouse Thymus Tissue, Rat Ovary Tissue, Rat Colon Tissue, Rat Thymus Tissue, HepG2 Whole Cell, HeLa Whole Cell

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human FABP4 peptide (ab66681).

ターゲ��ト情報

  • 機能Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • 配列類似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメインForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 細胞内局在Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Anti-FABP4 antibody 画像

  • All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : P7 Mouse Adipose Tissue Lysate
    Lane 3 : Adult Rat Brown Adipose Tissue Lysate (ab48639)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 30 kDa,44 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab66682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 3 : Rat Ovary Tissue Lysate
    Lane 4 : Rat Colon Tissue Lysate
    Lane 5 : Rat Thymus Tissue Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 18 kDa (possible post-translational modification).

Anti-FABP4 antibody (ab66682) 使用論文

This product has been referenced in:
  • Roberts JN  et al. Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation. ACS Nano 10:6667-79 (2016). ICC/IF . Read more (PubMed: 27322014) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample Human Cell (Adipose-derived stem cells for adipogenesis)
Specification Adipose-derived stem cells for adipogenesis
Permeabilization Yes - 0.5% Triton X-100, room temperature 15 mintutes
Fixative Paraformaldehyde
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投稿 Mar 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"