製品の概要

  • 製品名Anti-FABP4 antibody
    FABP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FABP4
  • アプリケーション適用あり: WB, ELISA, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • 配列類似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメインForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 細胞内局在Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Anti-FABP4 antibody 画像

  • Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
  • Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-FABP4 antibody (ab13979) 使用論文

This product has been referenced in:
  • Ohyama Y  et al. Differential expression of fatty acid-binding proteins and pathological implications in the progression of tongue carcinoma. Mol Clin Oncol 2:19-25 (2014). IHC-P ; Mouse . Read more (PubMed: 24649302) »
  • Nobusue H  et al. Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation. Nat Commun 5:3368 (2014). Read more (PubMed: 24569594) »

See all 6 Publications for this product

Product Wall

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The antibody you have ordered is a rabbit polyclonal to FABP4 therefore, you will need to use an anti-rabbit secondary antibody so that it can recognise and bind to your primary antibody.

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Thank you for contacting Abcam regarding ab13979.

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for your enquiry. The immunogen used to make this antibody was not a peptide but the full length protein. SwissProt link (http://www.uniprot.org/uniprot/P15090) I have taken the liberty BLASTing the sequence with zebrafish to check f...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas and peri-pancreatic fatty tissue)
Specification pancreas and peri-pancreatic fatty tissue
Fixative 10% NBF
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step 10% goat serum/1%BSA in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Aug 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"