Anti-FABP4 抗体 (ab13979)
Key features and details
- Rabbit polyclonal to FABP4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FABP4 antibody
FABP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FABP4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.134% PBS, 0.58% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13979の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
1/200.
|
特記事項 |
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IHC-P
1/200. |
ターゲット情報
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機能
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
細胞内局在
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. - Information by UniProt
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参照データベース
- Entrez Gene: 2167 Human
- Omim: 600434 Human
- SwissProt: P15090 Human
- Unigene: 391561 Human
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別名
- 3T3-L1 lipid-binding protein antibody
- 422/aP2 antibody
- A-FABP antibody
see all
画像
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Immunohistochemical analysis of FFPE human adipose tissue, labeling FABP4 with ab13979 (2µg/ml). DAB staining.
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Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB. -
Immunohistochemical analysis of FFPE mouse pancreatic and peri-pancreatic fatty tissue, labeling FABP4 with ab13979. Tissue was fixed in 10% neutral buffered formalin, heat mediated antigen retrieval was performed using citrate buffer, and blocking was performed with 10% goat serum and 1% BSA inj TBST for 1 hour at 25°C.
Staining with ab13979 (diluted 1/500) was performed for 12 hours at 4°C. A goat anti-rabbit HRP-conjugated secondary was used, diluted 1/200.
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Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (37)
ab13979 は 37 報の論文で使用されています。
- Chen QY et al. The peroxisome proliferator-activated receptor agonist rosiglitazone specifically represses tumour metastatic potential in chromatin inaccessibility-mediated FABP4-deficient gastric cancer. Theranostics 12:1904-1920 (2022). PubMed: 35198079
- Dello Spedale Venti M et al. Morphological and Immunophenotypical Changes of Human Bone Marrow Adipocytes in Marrow Metastasis and Myelofibrosis. Front Endocrinol (Lausanne) 13:882379 (2022). PubMed: 35757418
- Miao L et al. FABP4 deactivates NF-?B-IL1a pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. Clin Transl Med 11:e395 (2021). PubMed: 33931964
- Jiang Z et al. PDGFRb+ mesenchymal cells, but not NG2+ mural cells, contribute to cardiac fat. Cell Rep 34:108697 (2021). PubMed: 33535029
- Mereuta OM et al. Characterization of the 'White' Appearing Clots that Cause Acute Ischemic Stroke. J Stroke Cerebrovasc Dis 30:106127 (2021). PubMed: 34592611