製品の概要

  • 製品名Anti-FAAP24 antibody
    FAAP24 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FAAP24
  • アプリケーション適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FAAP24.

    (Peptide available as ab49605.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa Nuclear extract, Jurkat Nuclear extract and Irradiated HeLa whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).

ターゲット情報

  • 関連性FAAP24 was identified as a protein that interacts with FANCM as part of the core Fanconi Anemia complex. It is homologous to the XPF family of endonucleases. FAAP24 is required for normal levels of FANCD2 ubiquitination after DNA damage. Knockdown of FAAP24 leads to chromosomal instability.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • C19ORF40 antibody
    • C19orf40 chromosome 19 open reading frame 40 antibody
    • FAAP24 antibody
    • Fanconi anemia-associated protein 24 kDa antibody
    • Fanconi anemia-associated protein of 24 kDa antibody
    • FAP24_HUMAN antibody
    • MGC32020 antibody
    see all

Anti-FAAP24 antibody 画像

  • All lanes : Anti-FAAP24 antibody (ab48124) at 1 µg/ml

    Lane 1 : Jurkat nuclear extract lysate (ab14844)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,40 kDa. We are unsure as to the identity of these extra bands.

Anti-FAAP24 antibody (ab48124) 使用論文

ab48124 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (various incl: HeLa, HEK293, U2OS)
Loading amount 30 µg
Specification various incl: HeLa, HEK293, U2OS
Gel Running Conditions Reduced Denaturing (12.5% SDS PAGE)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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投稿 Dec 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"