Rabbit polyclonal to FAAP24
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FAAP24.
(Peptide available as
This antibody gave a positive signal in HeLa Nuclear extract, Jurkat Nuclear extract and Irradiated HeLa whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
FAAP24 was identified as a protein that interacts with FANCM as part of the core Fanconi Anemia complex. It is homologous to the XPF family of endonucleases. FAAP24 is required for normal levels of FANCD2 ubiquitination after DNA damage. Knockdown of FAAP24 leads to chromosomal instability.
C19orf40 chromosome 19 open reading frame 40 antibody
Western blot - Anti-FAAP24 antibody (ab48124)
All lanes :
Anti-FAAP24 antibody (ab48124) at 1 µg/ml
Lane 1 : Jurkat nuclear extract lysate ( ab14844) Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Predicted band size: 24 kDa Observed band size: 25 kDa ( why is the actual band size different from the predicted?) Additional bands at: 120 kDa, 40 kDa. We are unsure as to the identity of these extra bands. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
has not yet been referenced specifically in any publications.
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