This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate; Small Intestine (Human) Tissue Lysate; Pancreas (Human) Tissue Lysate; Skeletal Muscle (Human) Tissue Lysate; Testis (Human) Tissue Lysate; A431 Whole Cell Lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 mg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.
Belongs to the amidase family.
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.
All lanes : Anti-FAAH1 antibody (ab98065) at 1 µg/ml
Lane 1 : Human small intestine tissue lysate - total protein (ab29276) Lane 2 : Liver (Mouse) Nuclear Lysate - normal tissue (ab29300) Lane 3 : Human testis tissue lysate - total protein (ab30257) Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 63 kDa Observed band size : 63 kDa Additional bands at : 20 kDa,25 kDa,40 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab98065 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SKNSH cells at 5µg/ml.