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RabMAb

Anti-F4/80 抗体 [SP115] (ab111101)

製品の概要

  • 製品名
    Anti-F4/80 antibody [SP115]
    F4/80 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP115] to F4/80
  • アプリケーション
    適用あり: IHC-P, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from the N terminus of Mouse F4/80 protein.

  • ポジティブ・コントロール
    • Mouse liver tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450

ターゲット情報

  • 機能
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性
    Expression is restricted to eosinophils.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adgre1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [SP115] 画像

  • ab111101 staining F4/80 in Mouse uterus and spleen (multi block) tissue sections

    See Abreview

  • ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.

  • Immunohistochemistry analysis of Formalin fixed paraffin-embedded mouse lung tissue sections labeling F4/80 with ab111101 at 1/200 for 16 hours at 4°C. Biotin conjugated Goat anti-rabbit polyclonal antibody at 1/500 was used as the secondary. Antigen retrieval was heat mediated using citrate buffer pH 6.0. 

    See Abreview

プロトコール

Anti-F4/80 antibody [SP115] (ab111101) 使用論文

This product has been referenced in:
  • Nagashima H  et al. CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice. Mol Pain 13:1744806916689269 (2017). Read more (PubMed: 28326928) »
  • Sheng ZY  et al. Sertoli Cells Are Susceptible to ZIKV Infection in Mouse Testis. Front Cell Infect Microbiol 7:272 (2017). Read more (PubMed: 28680856) »

See all 12 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (uterus and spleen (multi-block))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
Permeabilization
No
Specification
uterus and spleen (multi-block)
Blocking step
Vector casein solution as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Henry King

Verified customer

投稿 Aug 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative
10% NBF
Username

Abcam user community

Verified customer

投稿 Jul 11 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (rat blood vessels)
Antigen retrieval step
None
Permeabilization
No
Specification
rat blood vessels
Blocking step
Dako blocking solution as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 01 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Permeabilization
No
Specification
lung
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application
Flow Cytometry
Sample
Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization
No
Gating Strategy
CD45+ cells
Specification
Leukocytes from lung (bronchoalveolar lavage)
Preparation
Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Fixation
None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application
Immunocytochemistry
Sample
Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization
No
Specification
Leukocytes from lung (bronchoalveolar lavage)
Fixative
Dehydrated cells by Cytospin
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization
No
Specification
lung
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Macrophages from Bronchoalveolar lavage)
Permeabilization
No
Specification
Macrophages from Bronchoalveolar lavage
Fixative
None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

投稿 Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 23 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Infarcted mouse heart)
Specification
Infarcted mouse heart
Permeabilization
No
Fixative
Acetone
Username

Nick

Verified customer

投稿 Jul 15 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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