製品の概要

  • 製品名Anti-F4/80 antibody [SP115]
    F4/80 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP115] to F4/80
  • アプリケーション適用あり: IHC-P, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from the N terminus of Mouse F4/80 protein.

  • ポジティブ・コントロール
    • Mouse liver tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min. This product is sensitive to tissue ageing and use of fresh cut sections is recommended.

IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450

ターゲット情報

  • 機能Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性Expression is restricted to eosinophils.
  • 配列類似性Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [SP115] 画像

  • ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.

Anti-F4/80 antibody [SP115] (ab111101) 使用論文

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Permeabilization No
Specification lung
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application Flow Cytometry
Sample Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization No
Gating Strategy CD45+ cells
Specification Leukocytes from lung (bronchoalveolar lavage)
Preparation Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Fixation None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application Immunocytochemistry
Sample Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization No
Specification Leukocytes from lung (bronchoalveolar lavage)
Fixative Dehydrated cells by Cytospin
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization No
Specification lung
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Dec 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Macrophages from Bronchoalveolar lavage)
Permeabilization No
Specification Macrophages from Bronchoalveolar lavage
Fixative None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

投稿 Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 23 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (Infarcted mouse heart)
Specification Infarcted mouse heart
Permeabilization No
Fixative Acetone
Username

Nick

Verified customer

投稿 Jul 15 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Mouse Tissue sections (bone)
Specification bone
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 24 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Sample Mouse Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 16 2013


The recommended antigen retrieval buffer is 1 mM EDTA solution, pH 8, in water.

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