製品の概要

  • 製品名
    Anti-F4/80 antibody [F4/80]
    F4/80 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [F4/80] to F4/80
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Thioglycollate stimulated peritoneal macrophages from C57/BL mice

  • ポジティブ・コントロール
    • Mouse liver tissue lysate; MCF7; HeLa ICC/IF: Raw 264.7 cells
  • 特記事項

    This antibody clone is manufactured by Abcam.

     

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    F4/80
  • ミエローマ
    NS1
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 98 kDa).

ターゲット情報

  • 機能
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性
    Expression is restricted to eosinophils.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

画像

  • Ab90247 stained Raw 264.7 cells. The cells were 100% methanol fixed (5 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Triton for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90247 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-F4/80 antibody [F4/80] (ab90247) at 10 µg/ml + Liver (Mouse) Tissue Lysate at 20 µg

    Secondary
    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) (ab88508) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 98 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,130 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutesF4/80 protein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

参考文献

This product has been referenced in:
  • Hume DA  et al. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J Exp Med 158:1522-36 (1983). Read more (PubMed: 6355361) »
  • Austyn JM & Gordon S F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol 11:805-15 (1981). Read more (PubMed: 7308288) »

See all 2 Publications for this product

レビューと Q&A

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録