製品の概要

  • 製品名Anti-F4/80 antibody [CI:A3-1]
    F4/80 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [CI:A3-1] to F4/80
  • 特異性This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages. Abcam does not recommend this product for IHC-P.
  • アプリケーション適用あり: WB, IP, RIA, IHC-Fr, Flow Cyt, ICC, IHC-FoFr, ICC/IF, IHC-Rmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Thioglycollate stimulated peritoneal macrophages from C57/BL mice

  • ポジティブ・コントロール
    • Mouse spleen and macrophages ICC/IF: Raw264.7 cells.
  • 特記事項

    Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.60
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名CI:A3-1
  • ミエローマNS1
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Use under non reducing condition. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa). (predicted molecular weight of precursor protein: 102 kDa; protein is heavily glycosylated). Block in 5% milk for 1 hour.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use a concentration of 10 µg/ml.
Flow Cyt 1/10 - 1/50.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 5 - 10 µg/ml.
IHC-R Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性Expression is restricted to eosinophils.
  • 配列類似性Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞��局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [CI:A3-1] 画像

  • ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-F4/80 antibody [CI:A3-1] (ab6640) at 1/1000 dilution

    Lane 1 : Mouse macrophage lysates
    Lane 2 : Mouse hepatocyte lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP labelled donkey anti-rat at 1/5000 dilution

    Predicted band size : 102 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab6640 staining F4/80 in mouse spleen tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 45 minutes at 21°C. The sample was incubated with primary antibody (1/100 in PBS + 5% FBS) at 4°C for 16 hours. An Alexa Fluor® 488-conjugated goat anti-rat IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody.

    See Abreview

  • Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

  • Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

  • Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

  • ab6640 staining F4/80 (red) in Mouse intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/10µg/ml in PBS + 10% serum) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rat IgG polyclonal (1/1000) was used as the secondary antibody. Blue - nuclei.

    See Abreview

Anti-F4/80 antibody [CI:A3-1] (ab6640) 使用論文

This product has been referenced in:
  • Doi H  et al. Potency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep 6:18844 (2016). IHC ; Mouse . Read more (PubMed: 26728342) »
  • Shiokawa M  et al. Pathogenicity of IgG in patients with IgG4-related disease. Gut N/A:N/A (2016). Human . Read more (PubMed: 26964842) »

See all 129 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Oct 11 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization No
Specification spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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投稿 Aug 19 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Jaculus jaculus Tissue sections (spleen)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteaseK
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Mai Tran

Verified customer

投稿 Jul 12 2016

Application Western blot
Sample Human Cell lysate - whole cell (Human Macrophages)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification Human Macrophages
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 Apr 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative 10% formalin
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投稿 Feb 18 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
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投稿 Oct 14 2015

Application Immunocytochemistry
Sample Mouse Cultured Cells (peritoneal macrophages)
Permeabilization No
Specification peritoneal macrophages
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Varkha Agrawal

Verified customer

投稿 Jun 05 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Antigen retrieval step None
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Paraformaldehyde
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投稿 Jan 05 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 30 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"