製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to F4/80
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fragment corresponding to Human F4/80 aa 626-859.
    Database link: Q14246

  • ポジティブ・コントロール
    • A431, H1299 whole cell lysates

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 98 kDa.

ターゲット情報

  • 機能
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性
    Expression is restricted to eosinophils.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

画像

  • All lanes : Anti-F4/80 antibody (ab96641) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 98 kDa

プロトコール

参考文献

ab96641 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (peritoneal macrophage)
Specification
peritoneal macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 12 2014


**Please note that you need to purchase this vial of ab74383 at list price as you normally would. After you submit your Abreview describing your experience staining rat tissue with ab74383, you can then use the discount code above to get your free...

Read More

Thanks for your email.
ab96641 may work in histo immuno staining but this application has not yet been tested.
I hope this helps. Please contact me if you have any further questions.
Use our products? Submit an Abreview. Earn rewards! Read More

Thank you for your enquiry.
Yes, ab74383 and ab15694 both stain F4/80. ab74383 has been found to react with rat F4/80 using western blotting.
I hope this helps. Please contact me again if you have any further questions.

Thank you for your enquiry.
We have not done side by side comparisons using these two antibodies.
Please contact me if you have any further questions.

Sure, ab74383 is also a good option for testing in the promotion.
Would you like me to arrange the testing discount promotion code for testing ab74383 with your rat sections?

Thank you for your enquiry.
At this time we don't have any antibodies that have been tested for reactivity to rat F4/80 AND have been used in IHC.
You might consider ab15694, a biotinilated rat antibody that has been used successfully in IHC....

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録