F-actin Staining Kit - Red Fluorescence | Cytopainter (ab112127)

製品の概要

  • 製品名F-actin Staining Kit - Red Fluorescence | Cytopainter
    F-actin キット 製品一覧
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプCell-based
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mammal
  • 製品の概要

    F-actin Staining Kit - Red Fluorescence | Cytopainter (ab112127) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    ab112127 is designed to stain F-actins in fixed cells with red fluorescence. The red fluorescent phalloidin conjugate, which is selectively bound to F-actins, is a high-affinity probe for F-actins. The red fluorescent phalloidin conjugate has Ex/Em = 594/610 nm. Used at nanomolar concentrations, phallotoxins can be conveniently used to label, identify and quantitate F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The red fluorescent phalloidin conjugate has good thermal and photo stability.


    ab112127 provides all the essential components with an optimized labeling protocol. It is an excellent tool for preserving fluorescent images of particular cells, and can also be used for fluorescence microscope demonstrations.

  • 特記事項

    ab112127 should be stored dessicated.

  • アプリケーション適用あり: FM, ICC/IFmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112127 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    F-actin Staining Kit - Red Fluorescence | Cytopainter 画像

    •  F-actin staining (red) in chondrocytes in growth plate cartilage. Mouse femur bone was fixed with 4% PFA for 72 hours, decalcified and cut into 8-10 µm sections in frozen. Tissue was then stained for 60 minutes with ab112127 before a final wash in PBS.

       

      This image is courtesy of an anonymous Abreview

    • HeLa cells were fixed with 4% PFA for 10 minutes, rinsed with PBS and stained for 60 minutes with ab112127 before a final wash in PBS prior to mounting slides.

      Images obtained with an Olympus BX61 microscope using a Texas Red filter (595/613nm) - 50ms exposure.

    • Images of CPA cells fixed with formaldehyde and stained with ab112127 in a black 96-well plate Left image: Cells labeled with 1X Red Fluorescent Phalloidin Conjugate for 30 min only. Right image: Cells treated with phalloidin for 10 min, then stained with Red Fluorescent Phalloidin Conjugate for 30 min.

    プロトコール

    F-actin Staining Kit - Red Fluorescence | Cytopainter (ab112127) 使用論文

    This product has been referenced in:
    • Kvirkvelia N  et al. Prostaglandin E2 promotes cellular recovery from established nephrotoxic serum nephritis in mice, prosurvival, and regenerative effects on glomerular cells. Am J Physiol Renal Physiol 304:F463-70 (2013). IHC . Read more (PubMed: 23283994) »

    See 1 Publication for this product

    Product Wall

    CytoPainter F-actin Staining Kit for IHC-P

    Poor Excellent 5/5 (Ease of Use)
    Abreviews
    I have tried this Kit on paraffin embedded sections of adult human kidney. Because the protocol is for ICC I adjusted it to IHC. In addition, I combined it with HLA staining (ab52922, routinely used at our lab) to visualize the tissue.

    Deparaffinization and antigen retrieval was done with commercial - ZYTOMED's OmniPrep (pH 9). Slides were washed with PBS-Tween (0.05%) and blocked (commercial - Invitrogen's CAS-Block) – 1h at RT. Anti-HLA was incubated for 1h at RT and washed with PBST three times.

    Phalloidin reagent was prepared according to protocol in three dilutions: 1/250, 1/500 and 1/1000. Secondary Alexa flour 488 donkey anti rabbit was added to each reagent tube at a 1/1200 dilution. Slides were incubated for 1h at RT and then washed three times with PBST. Secondary only slices (only Secondary Alexa flour 488) were used to determine exposure time threshold.
    There was no signal above background in all three Phalloidin dilutions. The picture (at the Texas Red Tfilter) was taken in slight overexposure to show the absent of staining.

    Username

    Abcam user community

    Verified customer

    投稿 Jul 10 2017

    Cytopainter F-actin in Hydractinia

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We used cytopainter F-actin staining kit to study morphology in the model organism Hydractinia during head regeneration. We fixed our whole animals in 4% pararfomaldehde in PBS followed by three ten minute washes in 0.1%triton in PBS. The animals were incubated in component in at a 1:500 dilution in component B for 45 minutes. They were then washed in PBS three times and mounted in fluorescent mounting medium.
    The picture shows F-actin staining(Red) and Hoescht nuclear staining(blue). The image was taken on a spinning disc confocal microscope.
    Username

    Mr. James Gahan

    Verified customer

    投稿 Jan 23 2014

    Yes, I believe they are also in the bone matrix.

    http://www.ncbi.nlm.nih.gov/pubmed/9610818.

    Yes, it does. Please see attached reference.





    http://www.ncbi.nlm.nih.gov/pubmed/9610818



    http://cjasn.asnjournals.org/content/3/Supplement_3/S131.full.pdf+html

    Thank you for contacting us.

    The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies...

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    Thank you for contacting us.

    This kit has an excitation of 583 nm and an emission at 603 nm. I have contacted the lab who confirm that this kit can be used with other antibodies providing that its emission does not overlap with the kits emiss...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for your reply and I am sorry about the confusion over the component list and the protocol.

    As you have added the Methanol to the already reconstituted Component A, I would be happy to send you a new kit, as I am sure if the Componen...

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    Thank you for contacting Abcam.

    I am sorry about the mix up, but it seems as though you received the older version of the protocol. Also, in the newer version of the kit, there is only Component A & B, and component A is already in a liqui...

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    Vielen Dank für Ihren Anruf.

    Ich freue mich Ihnen bestätigen zu können, dass die 50x Stock Solution von Red Fluorescent Phalloidin Conjugate für sechs Monate bei -20C aufgehoben werden kann.

    Ich hoffe diese Infor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"