製品の概要

  • 製品名
    Anti-F-actin antibody [NH3]
    F-actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NH3] to F-actin
  • 特異性
    Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-R, WB, ELISA, IHC-Fr, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Human monocytes and U397 cell line.

  • エピトープ
    This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
  • ポジティブ・コントロール
    • gastrointestinal tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-R Use at an assay dependent concentration.
WB 1/100 - 1/500. Detects a band of approximately 43 kDa. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
ELISA 1/10.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 27336173

ターゲット情報

画像

  • Anti-F-actin antibody [NH3] (ab205) at 1/100 dilution + Human pancreatic cancer cell line, whole cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-mouse antibody at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of ARPE cells labelling F-actin with ab205. Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS, and blocked for one hour. Cells were then incubated for 3h at 37°C with Anti-F-actin antibody [NH3] (ab205) at 1/200 dilution. Thereafter, cells were washed 3 times with PBS containing 0.3% Triton-X, incubated with secondary antibody and coverslipped with Fluoroshield containing DAPI as a counter stain. Immunofluorescent signals were detected by confocal microscopy.

  • Immunohistochemistry (Resin sections) analysis of mouse bone tissue cells labeling F-actin with ab205 at 1/200 dilution. Mouse tibia bone samples were fixed in neutral buffered formalin for 72 hours at room temperature, embedded undecalcified in plastic and sectioned at 5 microns.  A polyclonal goat anti-mouse Alexa Fluor® 488 secondary antibody was used at 1/700 dilution. The image shows a 1 micron thick z-projection of the chondrocytes in the proximal tibia growth plate. 

    See Abreview

  • Anti-F-actin antibody [NH3] (ab205) at 1/500 dilution + CNS whole tissue lysate at 30 µg

    Secondary
    Goat anti-mouse HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous abreview.

    Blocking: 5% milk for 1 hour at 25°C. 

    Incubation with primary antibody for 15 hours at 4°C in 5%milk+ PBS+0.1% Tween 20.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of mouse cortical neurons labeling F-actin with ab205 at 1/100 dilution. The cells were fixed with Ethanol and permeabilized with 0.2% Triton X-100. A polyclonal goat anti-mouse Cy3 conjugated secondary antibody was used at 1/200 dilution. 

    See Abreview

  • ab205 staining F-actin in rat brain tissue by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 2% BSA for 1 hour followed by incubation with the primary antibody at a 1/25 dilution for 24 hours. A biotin-conjugated goat anti-mouse IgM was used as secondary antibody at a 1/400 dilution. No permeabilisation step was used.

参考文献

This product has been referenced in:
  • Wang A  et al. Tumor-associated macrophages promote Ezrin phosphorylation-mediated epithelial-mesenchymal transition in lung adenocarcinoma through FUT4/LeY up-regulation. Oncotarget 8:28247-28259 (2017). IF . Read more (PubMed: 28423676) »
  • Chen X  et al. Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition. Tumour Biol 39:1010428317695942 (2017). Read more (PubMed: 28351308) »

See all 22 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Derived Breast cancer Cell Line)
Permeabilization
Yes - 0.5 % Triton
Specification
Mouse Derived Breast cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Fixative
Methanol
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投稿 Aug 17 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Derived Breast Cancer Primary Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Mouse Derived Breast Cancer Primary Cell Line
Blocking step
Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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投稿 Aug 14 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cortical Neurons)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Cortical Neurons
Blocking step
casein solution + Tween 20 as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Ethanol
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Verified customer

投稿 Aug 04 2016

Je peux vous proposer ab205, Anti-F-actin antibody [NH3] qui est un monoclonal de souris garanti de fonctionner en immunofluorescence. Cependant, nous n’avons pas testé le taux de toxine dans ce produit et donc nous ne pouvons pas garantir...

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Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Tissue lysate - whole (CNS)
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Aug 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Resin sections)
Sample
Mouse Tissue sections (Bone Tissue Cells)
Specification
Bone Tissue Cells
Username

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Verified customer

投稿 Jul 10 2013

Thank you for your message and for providing this further information.
I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrang...

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Thank you for taking the time to provide additional information and to include our suggestions in your experiments. I appreciate the time that was spent doing additional experiments. I am sorry to hear that the results obtained are still not satisfacto...

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Thank you for contacting us.
I am sorry to hear you have been experiencing problems with one of our Anti-F-actin antibody [NH3] (ab205). The quality of our products is important to us and I would like to reassure you that we investigate all custom...

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Thank you for contacting us.

Unfortunately, we have not determined the pI (isoelectric) value for this product, ab205 Anti-F-actin antibody [NH3], nor any relevant goat anti-mouse IgG conjugated FITC antibodies like ab97022 and ab97039.
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1-10 of 33 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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