製品の概要

  • 製品名Anti-Ezrin (phospho Y478) antibody
    Ezrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ezrin (phospho Y478)
  • 特異性Binds to Ezrin only when phosphorylated at tyrosine 478.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Ezrin around the phosphorylation site of tyrosine 478 (P-V-YP-E-P)

  • ポジティブ・コントロール
    • Extracts form HeLa cells treated with Na3VO4 (0.3nM, 40mins).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.
ELISA 1/5000.

ターゲット情報

  • 機能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 組織特異性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • 配列類似性Contains 1 FERM domain.
  • 発生段階Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • 翻訳後修飾Phosphorylated by tyrosine-protein kinases.
  • 細胞内局在Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
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    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
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    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin (phospho Y478) antibody 画像

  • All lanes : Anti-Ezrin (phospho Y478) antibody (ab79256) at 1/500 dilution

    Lane 1 : K562 cell lysate treated with Na3VO4 (0.3nM, 40mins)
    Lane 2 : K562 cell lysate treated with Na3VO4 (0.3nM, 40mins) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 69 kDa
    Observed band size : 69 kDa

Anti-Ezrin (phospho Y478) antibody (ab79256) 使用論文

ab79256 has not yet been referenced specifically in any publications.

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