製品の概要

  • 製品名Anti-Ezrin (phospho Y146) antibody
    Ezrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ezrin (phospho Y146)
  • 特異性Detects endogenous levels of Ezrin only when phosphorylated at Tyrosine 146.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Ezrin around the phosphorylation site of Tyrosine 146 (SGYPLS).

  • ポジティブ・コントロール
    • Extracts from UV-treated 293 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 69 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • 組織特異性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • 配列類似性Contains 1 FERM domain.
    • 発生段階Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • 翻訳後修飾Phosphorylated by tyrosine-protein kinases.
    • 細胞内局在Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Anti-Ezrin (phospho Y146) antibody 画像

    • All lanes : Anti-Ezrin (phospho Y146) antibody (ab92557) at 1/500 dilution

      Lane 1 : 293 cells (treated with UV for 15 mins)
      Lane 2 : 293 cells (treated with UV for 15 mins) with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 69 kDa

    Anti-Ezrin (phospho Y146) antibody (ab92557) 使用論文

    ab92557 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92557.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"