製品の概要

  • 製品名
    Anti-Ezrin (phospho T567) antibody
    Ezrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ezrin (phospho T567)
  • 特異性
    This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Ssynthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 567 (Y-K-TP-L-R)

  • ポジティブ・コントロール
    • Extracts from A431 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA 1/20000.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 組織特異性
    Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • 配列類似性
    Contains 1 FERM domain.
  • 発生段階
    Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • 翻訳後修飾
    Phosphorylated by tyrosine-protein kinases.
  • 細胞内局在
    Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin (phospho T567) antibody 画像

  • All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)

    Lane 1 : EGF treated A431 cells, no added peptide
    Lane 2 : Untreated A431 cells, no added peptide


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b
  • All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)

    Lane 1 : Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)
    Lane 2 : Jurkat cell lysate


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : 68 kDa (possible cross reactivity).The lower band is likely to be Moesin, which has a homologous immunogenic region.

Anti-Ezrin (phospho T567) antibody (ab47293) 使用論文

This product has been referenced in:
  • Cicvaric A  et al. The brain-tumor related protein podoplanin regulates synaptic plasticity and hippocampus-dependent learning and memory. Ann Med 48:652-668 (2016). WB ; Mouse . Read more (PubMed: 27558977) »
  • Miura S  et al. Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6. Nat Commun 6:8871 (2015). WB ; Human . Read more (PubMed: 26563429) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (whole worm lysates)
Gel Running Conditions
Non-reduced Denaturing (10% T gel concentration)
Loading amount
30000 cells
Specification
whole worm lysates
Blocking step
Roti Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Jun 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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