製品の概要

  • 製品名
    Anti-Ezrin antibody [EP924Y]
    Ezrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP924Y] to Ezrin
  • 由来種
    Rabbit
  • 特異性
    ab75840 detects both phosphorylated and non phosphorylated Ezrin.
  • アプリケーション
    適用あり: WB, ICC, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Ezrin (phospho Y145). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • 293, A431 and SH-SY5Y cell lysates.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能
      Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • 組織特異性
      Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • 配列類似性
      Contains 1 FERM domain.
    • 発生段階
      Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • 翻訳後修飾
      Phosphorylated by tyrosine-protein kinases.
    • 細胞内局在
      Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Ezrin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HCT116 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab75840 observed at 75 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab75840 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in Ezrin knockout cells. Wild-type and Ezrin knockout samples were subjected to SDS-PAGE. Ab75840 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/10000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 69 kDa
      Observed band size: 81 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Röwer C  et al. Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues. Transl Oncol 11:1-10 (2017). WB ; Human . Read more (PubMed: 29132012) »
    • Watanabe K  et al. Participation of ezrin in bacterial uptake by trophoblast giant cells. Reprod Biol Endocrinol 7:95 (2009). Read more (PubMed: 19737422) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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