ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P or IP.
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
Contains 1 FERM domain.
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
Phosphorylated by tyrosine-protein kinases.
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: Ezrin knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: HCT116 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75840 observed at 75 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab75840 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in Ezrin knockout cells. Wild-type and Ezrin knockout samples were subjected to SDS-PAGE. Ab75840 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Ezrin antibody [EP924Y] (ab75840)
All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/10000 dilution
Lane 1 : 293 cell lysate Lane 2 : A431 cell lysate Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : goat anti-rabbit HRP at 1/2000 dilution
Röwer C et al. Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues. Transl Oncol11:1-10 (2017).
Read more (PubMed: 29132012) »