製品の概要

  • 製品名Anti-Ezrin antibody
    Ezrin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Ezrin
  • 特異性Cross-reactivity with Radixin and Moesin has not been tested.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human Ezrin protein (AAH13903) with a 26 kDa tag.

  • ポジティブ・コントロール
    • Immunogen, HepG2 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 組織特異性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • 配列類似性Contains 1 FERM domain.
  • 発生段階Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • 翻訳後修飾Phosphorylated by tyrosine-protein kinases.
  • 細胞内局在Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
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    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
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    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin antibody 画像

  • Anti-Ezrin antibody (ab76872) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 69 kDa
    Observed band size : 91 kDa (why is the actual band size different from the predicted?)
  • Anti-Ezrin antibody (ab76872) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

Anti-Ezrin antibody (ab76872) 使用論文

ab76872 has not yet been referenced specifically in any publications.

Product Wall

The cross-reactivity has not been tested. As the epitope is undetermined it is hard to say whether there is cross -reactivity. Whole protein homologies: EZRIN MOESIN 73 % EZRIN RADIXIN 76 % MOESIN RADIXIN 81 %

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"