製品の概要
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製品名Anti-Ezrin antibody
Ezrin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Ezrin
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アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Chicken
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Ezrin.
(Peptide available as ab42317.)
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ポジティブ･コントロール
- ab41672 gave a positive result in the following whole cell lysates: Hela, Jurkat, A431, HEK293, HepG2, MCF7, U20S.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab41672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa). | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
ターゲット情報
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機能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
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組織特異性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
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配列類似性Contains 1 FERM domain.
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発生段階Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
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翻訳後修飾Phosphorylated by tyrosine-protein kinases.
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細胞内局在Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
- Information by UniProt
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参照データベース
- Entrez Gene: 395701 Chicken
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 100008846 Rabbit
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
see all -
別名
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
Anti-Ezrin antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody (ab41672)This image is courtesy of an anonymous Abreview.ab41672 staining Ezrin in undifferentiated human embryonic stem cells on a murine embryonic fibroblast (MEFs) feeding layer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized and blocked with 1% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/250 in 1% serum, 0.1% triton, 0.1% BSA + PBS) for 16 hours at 4°C. An Alexa Flour® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/500.
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Western blot - Ezrin antibody (ab41672)All lanes : Anti-Ezrin antibody (ab41672) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)The observed molecular weight of 80 kDa is in agreement with previous findings (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482).
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Immunoprecipitation - Anti-Ezrin antibody (ab41672)Ezrin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal Ezrin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41672.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to anti-Rabbit HRP (IgG light chain) (ab99697).
Band: 80kDa: Ezrin -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ezrin antibody (ab41672)IHC image of Ezrin staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41672, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
Anti-Ezrin antibody (ab41672) 使用論文
This product has been referenced in:
- Horwitz V et al. Ezrin Is Associated with Disease Progression in Ovarian Carcinoma. PLoS One 11:e0162502 (2016). WB ; Human . Read more (PubMed: 27622508) »
- Perisic L et al. Schip1 is a novel podocyte foot process protein that mediates actin cytoskeleton rearrangements and forms a complex with Nherf2 and ezrin. PLoS One 10:e0122067 (2015). Read more (PubMed: 25807495) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"