製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EZH1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Synthetic peptide:

    EIPNPPTSKCITY

    , corresponding to amino acids 2-14 of Human EZH 1.

  • ポジティブ・コントロール
    • MOLT 4 lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EZH1 peptide (ab22954).

ターゲット情報

  • 機能
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • 配列類似性
    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • 細胞内局在
    Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    see all

画像

  • Anti-EZH1 antibody (ab3670) at 2 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 85 kDa
    Observed band size : 85 kDa

プロトコール

参考文献

This product has been referenced in:
  • Luo L  et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats. Exp Ther Med 13:645-650 (2017). Read more (PubMed: 28352345) »

See 1 Publication for this product

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