製品の概要

  • 製品名Anti-EYA4 antibody
    EYA4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EYA4
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from within residues: MEDSQDLNEQ SVKKTCTESD VSQSQNSRSM EMQDLASPHT LVGGGDTPGS, corresponding to N terminal amino acids 1-50 of Human EYA4 (NP_004091.3)

  • ポジティブ・コントロール
    • HeLa wholec cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye.
    • 組織特異性Highly expressed in heart and skeletal muscle.
    • 関連疾患Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10) [MIM:601316]. DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
      Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J) [MIM:605362]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • 配列類似性Belongs to the HAD-like hydrolase superfamily. EYA family.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CMD1J antibody
      • Deafness, autosomal dominant 10 antibody
      • DFNA 10 antibody
      • DFNA10 antibody
      • dJ78N10.1 (eyes absent (Drosophila) homolog 4) antibody
      • dJ78N10.1 (eyes absent) antibody
      • EYA 4 antibody
      • eya4 antibody
      • EYA4_HUMAN antibody
      • Eyes absent 4 antibody
      • Eyes absent homolog 4 (Drosophila) antibody
      • Eyes absent homolog 4 antibody
      • HGNC:3522 antibody
      • OTTHUMP00000040267 antibody
      see all

    Anti-EYA4 antibody 画像

    • All lanes : Anti-EYA4 antibody (ab93865) at 0.4 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 70 kDa


      Exposure time : 3 minutes
    • 3µg ab93865 were used to immunoprecipitate EYA4 from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with 1µg/ml ab93865.
      Detection: chemiluminescence with exposure time of 10 seconds.

    Anti-EYA4 antibody (ab93865) 使用論文

    ab93865 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab93865.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"