製品の概要

  • 製品名Anti-EYA3 antibody
    EYA3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EYA3
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human EYA3 aa 1-30.
    Database link: Q99504

  • ポジティブ・コントロール
    • HeLa, 293T cell lysate, IHC controls:breast, colon and prostate carcinoma

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab95876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/500.

ターゲット情報

  • 機能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
  • 配列類似性Belongs to the HAD-like hydrolase superfamily. EYA family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
  • 細胞内局在Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI844637 antibody
    • DKFZp686C132 antibody
    • EYA3 antibody
    • EYA3_HUMAN antibody
    • Eyes absent 3 antibody
    • Eyes absent 3 homolog (Drosophila) antibody
    • Eyes absent homolog 3 antibody
    • OTTMUSP00000010370 antibody
    • RP11-460I13.4 antibody
    • RP23-442A20.1 antibody
    see all

Anti-EYA3 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA3 with ab95876 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EYA3 antibody (ab95876) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 63 kDa


    Exposure time : 30 seconds
  • Detection of human Eya3 by Western Blot of Immunoprecipitate. ab95876 at 0.04µg/ml staining Eya3 in HeLa cell lysate immunoprecipitated using ab95876 at 3µg/mg (1mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-EYA3 antibody (ab95876) 使用論文

ab95876 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (Human Retinal Microvascular Enothelial)
Specification Human Retinal Microvascular Enothelial
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
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投稿 Jul 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"