製品の概要

  • 製品名Anti-EYA2 antibody
    EYA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EYA2
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 100 and 150 of human EYA2 (NP_005235.3)

  • ポジティブ・コントロール
    • 293T cells lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
  • 組織特異性Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
  • 配列類似性Belongs to the HAD-like hydrolase superfamily. EYA family.
  • 発生段階At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EAB 1 antibody
    • EAB1 antibody
    • EYA 2 antibody
    • EYA2 antibody
    • EYA2_HUMAN antibody
    • eyes absent 2 homolog (Drosophila) antibody
    • Eyes absent homolog 2 (Drosophila) antibody
    • Eyes absent homolog 2 antibody
    • Translation of this uORF probably lowers the translation efficiency of EYA2 antibody
    see all

Anti-EYA2 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA2 with ab95875 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EYA2 antibody (ab95875) at 0.2 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 59 kDa


    Exposure time : 30 seconds
  • Detection of human Eya2 by Western Blot of Immunoprecipitate. ab95875 at 0.1µg/ml staining Eya2 in 293T cell lysate immunoprecipitated using ab95875 at 3µg/mg (1mg/IP; 20% of IP loaded/lane) Detection: Chemiluminescence with exposure time of 10 seconds..

Anti-EYA2 antibody (ab95875) 使用論文

ab95875 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees t...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"