EXPOSE Mouse and Rabbit Specific HRP/AEC Detection IHC Kit (ab93686)

製品の概要

  • 製品名EXPOSE Mouse and Rabbit Specific HRP/AEC Detection IHC Kit
  • 検出方法Colorimetric
  • アッセイタイプMicro-Polymer
  • 製品の概要

    The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a secondary antibody-HRP conjugate which reacts with the primary antibody, and substrate-chromogen (AEC).

  • アプリケーション適用あり: IHC-Pmore details

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 15 ml 60 ml 125 ml
    AEC Single Solution 1 x 15ml 1 x 60ml 1 x 125ml
    HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml
    Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml
    Protein block 1 x 15ml 1 x 60ml 1 x 125ml
  • 研究分野
  • 関連性Biotin-Free Polyvalent AEC detection is a biotin-free immunoenzymatic antigen detection system. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab93686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.

EXPOSE Mouse and Rabbit Specific HRP/AEC Detection IHC Kit 画像

  • Immunohistochemical staining of p53 in human colon adenocarcinoma with anti-p53 antibody at a dilution of 1/400 and detected using ab93686.

  • Immunohistochemical staining of smooth muscle actin in human small intestine with ab7817 at a dilution of 1/200 and detected using ab93686.

EXPOSE Mouse and Rabbit Specific HRP/AEC Detection IHC Kit (ab93686) 使用論文

This product has been referenced in:
  • Watanabe K  et al. The MET/Vascular Endothelial Growth Factor Receptor (VEGFR)-targeted Tyrosine Kinase Inhibitor Also Attenuates FMS-dependent Osteoclast Differentiation and Bone Destruction Induced by Prostate Cancer. J Biol Chem 291:20891-20899 (2016). Read more (PubMed: 27539855) »

See 1 Publication for this product

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Thank you for your phone call last week. I have heard back from the lab, and I am told that the AEC solution provided in the kit is not filtered.  I hope this information is helpful, but let me know if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"