製品の概要

  • 製品名Anti-EXOSC8 antibody
    EXOSC8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EXOSC8
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the C terminal region of human EXOSC8.

  • ポジティブ・コントロール
    • HL60 cell line lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 30 kDa.
ELISA 1/1000.

ターゲット情報

  • 機能Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.
  • 配列類似性Belongs to the RNase PH family.
  • 細胞内局在Cytoplasm. Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA421P11.3 antibody
    • CBP interacting protein 3 antibody
    • CIP3 antibody
    • EAP2 antibody
    • EC 3.1.13 antibody
    • EXOS8_HUMAN antibody
    • EXOSC8 antibody
    • Exosome complex component RRP43 antibody
    • Exosome complex exonuclease RRP43 antibody
    • Exosome component 8 antibody
    • OIP-2 antibody
    • OIP2 antibody
    • Opa interacting protein 2 antibody
    • Opa-interacting protein 2 antibody
    • OTTHUMP00000042274 antibody
    • p9 antibody
    • Ribosomal RNA processing protein 43 antibody
    • Ribosomal RNA processing protein 43, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 43 antibody
    • RP11 421P11.3 antibody
    • RRP43 antibody
    • Rrp43p antibody
    see all

Anti-EXOSC8 antibody 画像

  • Anti-EXOSC8 antibody (ab82290) at 1/50 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunofluorescence analysis of Human skin fibroblasts, staining EXOSC8 with ab82290.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1 % Triton-X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 5% serum) for 3 hours at 25°C. An Cy2®-conjugated donkey anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

Anti-EXOSC8 antibody (ab82290) 使用論文

ab82290 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - other (Human skin fibroblast)
Loading amount 40 µg
Specification Human skin fibroblast
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 21 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Dec 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"