製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to EXOSC4
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide: AGLELLSDQG YRVDGRRAGE LRKIQARMGV FAQADGSAYI EQGNTKALAV, corresponding to amino acids 2-51 of Human EXOSC4

  • ポジティブ・コントロール
    • Jurkat cell lysate. Human heart tissue. Human liver tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.

WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性
    EXOSC4 belongs to the RNase PH family. It is a component of the exosome 3'->5' exoribonuclease complex and is required for the 3' processing of the 7S pre-RNA to the mature 5.8S rRNA. It has a 3'-5' exonuclease activity.
  • 細胞内局在
    Cytoplasm. Nucleus, nucleolus.
  • 参照データベース
  • 別名
    • EXOSC 4 antibody
    • Exosome complex component RRP41 antibody
    • Exosome complex exonuclease RRP41 antibody
    • Exosome component 4 antibody
    • Exosome component Rrp41 antibody
    • FLJ20591 antibody
    • hRrp41p antibody
    • p12A antibody
    • Ribosomal RNA processing protein 41 antibody
    • RRP 41 antibody
    • RRP41 antibody
    • RRP41A antibody
    • Rrp41p antibody
    • SKI 6 antibody
    • SKI6 antibody
    • Ski6p antibody
    see all

画像

  • All lanes : Anti-EXOSC4 antibody (ab66672) at 2.5 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling EXOSC4 with ab66672 at 4-8µg/ml. Arrow indicate positively stained hepatocyte. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling EXOSC4 with ab66672 at 4-8µg/ml. Arrow indicate positively labelled myocardial cells. Magnification: 400X.

参考文献

ab66672 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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